Scholarlink

Colorado tick fever virus (CTFV) is a double-stranded RNA virus in the family Reoviridae. CTFV is the causative agent of Colorado tick fever (CTF), a febrile illness that can have mild to severe manifestations. CTFV is an underreported and underrecognized virus; similarly, the literature concerning neglected tick-borne viruses lacks standard protocols for viral research. Here we describe protocols and procedures for CTFV studies, including the quantification of the virus through viral plaque assay, generation and storage of CTFV stocks, and detection with reverse transcription-quantitative PCR (RT-qPCR).

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Colorado Tick Fever Virus: Quantification, Propagation, Storage, and Detection

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • Stephanie Beane,
  • Megan Burch,
  • Jeremy Bechelli

摘要

Colorado tick fever virus (CTFV) is a double-stranded RNA virus in the family Reoviridae. CTFV is the causative agent of Colorado tick fever (CTF), a febrile illness that can have mild to severe manifestations. CTFV is an underreported and underrecognized virus; similarly, the literature concerning neglected tick-borne viruses lacks standard protocols for viral research. Here we describe protocols and procedures for CTFV studies, including the quantification of the virus through viral plaque assay, generation and storage of CTFV stocks, and detection with reverse transcription-quantitative PCR (RT-qPCR).