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Peptides and peptide libraries are valuable probes in solid-phase binding assays and widely used in studies of protein–protein interactions. However, a high unspecific background can disturb or even inhibit a successful experiment. We describe an easy and flexible method for pre-purification of samples that is particularly well-suited for high-throughput processing of large numbers of samples.

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Facile High-Throughput Pre-purification of Biological Samples for Library-Scale Peptide Interaction Studies

  • 实验方案

  • 发表日期：

    2025年

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  • Ulrike Beutling,
  • Sven-Kevin Hotop,
  • Ronald Frank,
  • Mark Brönstrup

摘要

Peptides and peptide libraries are valuable probes in solid-phase binding assays and widely used in studies of protein–protein interactions. However, a high unspecific background can disturb or even inhibit a successful experiment. We describe an easy and flexible method for pre-purification of samples that is particularly well-suited for high-throughput processing of large numbers of samples.