Scholarlink

The analytical technique of choice for the separation, identification, and quantification of carotenoids is the high-performance liquid chromatography (HPLC) with UV/Vis detection. Here, we describe basic procedures for the preparation and extraction of carotenoids in human blood and faecal samples and their analysis using C18 and C30 columns. The methods detailed in this chapter describe the type and volume of extraction solvent used, the duration of the successive steps, and the identification and quantification system and some considerations for obtaining the best parameters of recovery, selectivity, and specificity.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Major Dietary Carotenoids in Human Blood and Faeces Samples: Extraction, Identification, and Quantification by HPLC-DAD

  • 章节

  • 发表日期：

    2025年

查看全文
  • Begoña Olmedilla-Alonso,
  • Elena Rodríguez-Rodríguez

摘要

The analytical technique of choice for the separation, identification, and quantification of carotenoids is the high-performance liquid chromatography (HPLC) with UV/Vis detection. Here, we describe basic procedures for the preparation and extraction of carotenoids in human blood and faecal samples and their analysis using C18 and C30 columns. The methods detailed in this chapter describe the type and volume of extraction solvent used, the duration of the successive steps, and the identification and quantification system and some considerations for obtaining the best parameters of recovery, selectivity, and specificity.