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Fecal contamination of food samples originating from both human and animal sources poses a significant public health risk. Traditional laboratory techniques require specialized equipment and skilled workers, including a time-consuming enrichment process that makes them unsuitable for field testing. Here we describe a rapid risk-assessment assay for fecal contamination by targeting Bacteroidales using loop-mediated isothermal amplification (LAMP). The assay allows for naked-eye observation of reactions, detecting as few as eight copies of Bacteroidales per cm2 on the surface of food samples within 50 min.

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Fecal Contamination Assessment Through Loop-Mediated Isothermal Amplification of Bacteroidales in Food Samples

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  • 发表日期：

    2025年

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  • Jiangshan Wang,
  • Mohit S. Verma

摘要

Fecal contamination of food samples originating from both human and animal sources poses a significant public health risk. Traditional laboratory techniques require specialized equipment and skilled workers, including a time-consuming enrichment process that makes them unsuitable for field testing. Here we describe a rapid risk-assessment assay for fecal contamination by targeting Bacteroidales using loop-mediated isothermal amplification (LAMP). The assay allows for naked-eye observation of reactions, detecting as few as eight copies of Bacteroidales per cm2 on the surface of food samples within 50 min.