Loop-mediated isothermal amplification (LAMP) allows for the rapid, efficient, and cost-effective amplification of nucleic acids. In this chapter, we propose an RT-LAMP protocol for the detection of hepatitis E virus (HEV), an enterovirus of public health significance. The process begins with the preparation of a master mix containing the appropriate primers. The reaction is then carried out at a constant temperature of 63 °C for 45 min. During this step, RNA is reverse transcribed into cDNA, which is subsequently amplified by specific enzymes. Following amplification, an enzymatic digestion is performed, and the resulting products are detected via electrophoresis. Alternatively, other detection methods can be employed, such as monitoring the accumulation of magnesium pyrophosphate, observing visual color changes, detecting fluorescent signals, or using real-time detection techniques.