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This protocol describes a rapid and sensitive method for detecting hepatitis A virus (HAV) in food samples using reverse transcription loop-mediated isothermal amplification (RT-LAMP). The technique targets the conserved 5′-UTR region of the HAV genome with six specific primers, enabling simultaneous reverse transcription and amplification at 62.5 °C for 60 min. This method offers a quick and reliable approach for HAV detection in food, supporting food safety monitoring and outbreak investigations.

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Hepatitis A Virus Detection Using the Loop-Mediated Isothermal Amplification-Based (LAMP) Technique in Foods

  • 章节

  • 发表日期：

    2025年

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  • Rafael Dorighello Cadamuro,
  • Mariana Alves Elois,
  • Nadine Yeramian,
  • Daniel Pérez-Alonso,
  • Gislaine Fongaro,
  • David Rodríguez-Lázaro

摘要

This protocol describes a rapid and sensitive method for detecting hepatitis A virus (HAV) in food samples using reverse transcription loop-mediated isothermal amplification (RT-LAMP). The technique targets the conserved 5′-UTR region of the HAV genome with six specific primers, enabling simultaneous reverse transcription and amplification at 62.5 °C for 60 min. This method offers a quick and reliable approach for HAV detection in food, supporting food safety monitoring and outbreak investigations.