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This protocol describes the procedure for transfecting HeLa KDind cells in 35-mm plates using Lipofectamine 3000 for efficient plasmid DNA delivery. The goal is to achieve high transfection efficiency for subsequent analysis, such as measuring luciferase activity.

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HeLa Transfections with Lipofectamine 3000

  • 实验方案

  • 发表日期：

    2025年

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  • Karuppusamy Arunachalam,
  • Puthanpura Sasidharan Sreeja

摘要

This protocol describes the procedure for transfecting HeLa KDind cells in 35-mm plates using Lipofectamine 3000 for efficient plasmid DNA delivery. The goal is to achieve high transfection efficiency for subsequent analysis, such as measuring luciferase activity.