This protocol outlines the procedure for transfecting HEK293 cells using the calcium phosphate method for plasmid DNA delivery. It includes steps for preparing cells, transfection, and medium changes.

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Transfection in HEK293 Cells

  • Karuppusamy Arunachalam,
  • Puthanpura Sasidharan Sreeja

摘要

This protocol outlines the procedure for transfecting HEK293 cells using the calcium phosphate method for plasmid DNA delivery. It includes steps for preparing cells, transfection, and medium changes.