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This protocol describes the procedure for generating competent bacterial cells (e.g., TOP10, DH5α) that can take up foreign DNA, such as plasmids, through transformation. The process involves treating bacteria with calcium chloride (CaCl2) to make their cell membranes more permeable to DNA. This is a critical step for molecular cloning applications.

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Generation of Competent Bacteria

  • 实验方案

  • 发表日期：

    2025年

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  • Karuppusamy Arunachalam,
  • Puthanpura Sasidharan Sreeja

摘要

This protocol describes the procedure for generating competent bacterial cells (e.g., TOP10, DH5α) that can take up foreign DNA, such as plasmids, through transformation. The process involves treating bacteria with calcium chloride (CaCl2) to make their cell membranes more permeable to DNA. This is a critical step for molecular cloning applications.