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The Protocol for Maintenance, Isolation, and Primary Culture of Ehrlich Tumor Cells outlines a method for isolating and culturing Ehrlich Ascites Tumor (EAT) cells, which are commonly used for research in cancer biology and pharmacology. The protocol includes procedures for obtaining the tumor cells from donor mice, isolating viable cells from the ascitic fluid, and establishing primary cell cultures. It also provides guidance for maintaining the cultures, including medium preparation, conditions for growth, and subculturing techniques to ensure cell viability and proliferation for experimental purposes.

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Protocol for Maintenance, Isolation, and Primary Culture of Ehrlich Tumor Cells

  • 实验方案

  • 发表日期：

    2025年

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  • Karuppusamy Arunachalam,
  • Puthanpura Sasidharan Sreeja

摘要

The Protocol for Maintenance, Isolation, and Primary Culture of Ehrlich Tumor Cells outlines a method for isolating and culturing Ehrlich Ascites Tumor (EAT) cells, which are commonly used for research in cancer biology and pharmacology. The protocol includes procedures for obtaining the tumor cells from donor mice, isolating viable cells from the ascitic fluid, and establishing primary cell cultures. It also provides guidance for maintaining the cultures, including medium preparation, conditions for growth, and subculturing techniques to ensure cell viability and proliferation for experimental purposes.