This protocol outlines the process for Alcian Blue staining of 5 dpf embryos fixed with paraformaldehyde (PFA). The procedure generally spans 1–2 days, depending on the staining intensity and sample size.

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Alcian Blue Staining Protocol

  • Karuppusamy Arunachalam,
  • Puthanpura Sasidharan Sreeja

摘要

This protocol outlines the process for Alcian Blue staining of 5 dpf embryos fixed with paraformaldehyde (PFA). The procedure generally spans 1–2 days, depending on the staining intensity and sample size.