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This protocol describes the procedure for obtaining protein extracts from 24-h post-fertilization (hpf) zebrafish embryos that are compatible with protein electrophoresis and Western blot analysis. The goal is to extract yolk proteins while minimizing their interference with the electrophoretic run and preventing any obstruction of protein detection following electrotransfer.

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Extraction of Yolk Proteins from 24 HPF Zebrafish Embryos

  • 实验方案

  • 发表日期：

    2025年

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  • Karuppusamy Arunachalam,
  • Puthanpura Sasidharan Sreeja

摘要

This protocol describes the procedure for obtaining protein extracts from 24-h post-fertilization (hpf) zebrafish embryos that are compatible with protein electrophoresis and Western blot analysis. The goal is to extract yolk proteins while minimizing their interference with the electrophoretic run and preventing any obstruction of protein detection following electrotransfer.