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Flow cytometry is a powerful technique used to measure various cellular parameters in individual sperm cells, such as calcium levels, pH, membrane potential, and vitality. This protocol outlines the procedures for analyzing mouse sperm cells using the BD Accuri C6+ flow cytometer, detailing the preparation of reagents, sample handling, and necessary controls. The cytometric analysis is based on fluorescence parameters using specific fluorophores tailored to the properties of the markers being studied.

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Flow Cytometry Protocol for Mouse Sperm Cells (BD Accuri C6+)

  • 实验方案

  • 发表日期：

    2025年

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  • Karuppusamy Arunachalam,
  • Puthanpura Sasidharan Sreeja

摘要

Flow cytometry is a powerful technique used to measure various cellular parameters in individual sperm cells, such as calcium levels, pH, membrane potential, and vitality. This protocol outlines the procedures for analyzing mouse sperm cells using the BD Accuri C6+ flow cytometer, detailing the preparation of reagents, sample handling, and necessary controls. The cytometric analysis is based on fluorescence parameters using specific fluorophores tailored to the properties of the markers being studied.