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This protocol outlines the procedure for DNA extraction and PCR genotyping of zebrafish using fin clipping. This method is commonly employed for genotyping mutant or transgenic zebrafish lines, enabling the identification of specific genetic modifications. The process involves the extraction of DNA from a small sample of fin tissue, followed by PCR amplification of target regions for genotyping.

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Zebrafish Genotyping by Fin Clipping

  • 实验方案

  • 发表日期：

    2025年

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  • Karuppusamy Arunachalam,
  • Puthanpura Sasidharan Sreeja

摘要

This protocol outlines the procedure for DNA extraction and PCR genotyping of zebrafish using fin clipping. This method is commonly employed for genotyping mutant or transgenic zebrafish lines, enabling the identification of specific genetic modifications. The process involves the extraction of DNA from a small sample of fin tissue, followed by PCR amplification of target regions for genotyping.