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Genomic DNA extraction from HEK 293 cells is a critical step for downstream applications such as PCR, sequencing, and genotyping. This protocol outlines the use of TRIzol reagent for efficient DNA extraction, followed by phase separation, ethanol precipitation, and DNA washing steps to obtain high-quality genomic DNA. The extracted DNA can then be quantified and assessed for integrity before use in molecular analyses.

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Extraction of Genomic DNA

  • 实验方案

  • 发表日期：

    2025年

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  • Karuppusamy Arunachalam,
  • Puthanpura Sasidharan Sreeja

摘要

Genomic DNA extraction from HEK 293 cells is a critical step for downstream applications such as PCR, sequencing, and genotyping. This protocol outlines the use of TRIzol reagent for efficient DNA extraction, followed by phase separation, ethanol precipitation, and DNA washing steps to obtain high-quality genomic DNA. The extracted DNA can then be quantified and assessed for integrity before use in molecular analyses.