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Cell sorting is a technique used to isolate specific cell populations based on unique characteristics, such as fluorescence. In this protocol, zebrafish embryos are sorted based on fluorescence markers to selectively isolate specific cell types. The procedure involves several key steps, including de-clustering, enzyme digestion, and centrifugation, to efficiently separate cells for downstream analysis.

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Cell Sorting of Fluorescent Zebrafish Cells

  • 实验方案

  • 发表日期：

    2025年

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  • Karuppusamy Arunachalam,
  • Puthanpura Sasidharan Sreeja

摘要

Cell sorting is a technique used to isolate specific cell populations based on unique characteristics, such as fluorescence. In this protocol, zebrafish embryos are sorted based on fluorescence markers to selectively isolate specific cell types. The procedure involves several key steps, including de-clustering, enzyme digestion, and centrifugation, to efficiently separate cells for downstream analysis.