Agarose gel electrophoresis is a widely used method to assess the integrity of RNA samples prior to downstream applications such as RT-PCR, RNA sequencing, or microarray analysis. The quality of RNA is critical for successful gene expression analysis, as degraded RNA can lead to unreliable results. This method involves separating RNA molecules based on their size and charge, with the intact ribosomal RNA (rRNA) bands, typically 28S and 18S rRNA, serving as indicators of RNA integrity. The presence of these intact bands suggests high-quality RNA, while the absence or smearing of these bands may indicate RNA degradation. The following protocol outlines the preparation of an agarose gel for RNA integrity assessment using SiberSafe nucleic acid stain for safe visualization under UV light.