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This protocol describes the procedure for extracting DNA and RNA from plasma samples using magnetized silica, specifically for detecting and quantifying HCV, HIV, HBV, and sexing-related markers. The extraction method utilizes silica-based magnetic beads to efficiently isolate nucleic acids, ensuring high-quality samples for downstream molecular analyses such as PCR and RT-PCR. This protocol provides step-by-step instructions for handling plasma samples, controlling contamination, and preparing samples for further genetic testing, including real-time PCR assays. Proper adherence to this procedure is crucial for obtaining reliable and accurate results.

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DNA and RNA Extraction from Plasma Using Magnetized Silica

  • 实验方案

  • 发表日期：

    2025年

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  • Karuppusamy Arunachalam,
  • Puthanpura Sasidharan Sreeja

摘要

This protocol describes the procedure for extracting DNA and RNA from plasma samples using magnetized silica, specifically for detecting and quantifying HCV, HIV, HBV, and sexing-related markers. The extraction method utilizes silica-based magnetic beads to efficiently isolate nucleic acids, ensuring high-quality samples for downstream molecular analyses such as PCR and RT-PCR. This protocol provides step-by-step instructions for handling plasma samples, controlling contamination, and preparing samples for further genetic testing, including real-time PCR assays. Proper adherence to this procedure is crucial for obtaining reliable and accurate results.