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This chapter presents a technical protocol for cellulase enzyme production via solid-state fermentation (SSF)Solid-state fermentation (SSF) using groundnutGroundnut (Arachis hypogea L.) shells, a lignocellulosic agro-waste, as a substrate. GroundnutGroundnut shells, containing 65.7% cellulose and 59.0% crude fiber, are pretreated using alkaline hydrolysis to disrupt lignocellulosic bonds and facilitate microbial access. Aspergillus niger, known for its cellulolytic capabilities, is used as a microbial agent. The chapter describes the preparation of spore suspensions, substrate sterilization, and inoculation processes to ensure optimal conditions for fungal growth. SSFSolid state fermentation (SSF) is conducted at 30 °C under controlled moisture and humidity conditions, enhancing enzyme–substrate interaction. Cellulase extraction involves buffer-mediated recovery, followed by centrifugation and filtration to isolate crude enzyme. Cellulase activity is quantified using a filter paper assay (FPA), with reducing sugars measured spectrophotometrically via the dinitrosalicylic acid (DNS) method. This methodology maximizes cellulase yield, aligning with sustainable bioresource valorization within the circular bioeconomy framework.

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Cellulase Enzyme Production Through Solid State Fermentation of Peanut Shells Using Microbial Cultures

  • 章节

  • 发表日期：

    2025年

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  • Nikhil Swaraj Arige,
  • Gayatri Dangeti,
  • Kandi Sridhar,
  • Minaxi Sharma,
  • Baskaran Stephen Inbaraj,
  • Praveen Kumar Dikkala,
  • Viharika Kondru

摘要

This chapter presents a technical protocol for cellulase enzyme production via solid-state fermentation (SSF)Solid-state fermentation (SSF) using groundnutGroundnut (Arachis hypogea L.) shells, a lignocellulosic agro-waste, as a substrate. GroundnutGroundnut shells, containing 65.7% cellulose and 59.0% crude fiber, are pretreated using alkaline hydrolysis to disrupt lignocellulosic bonds and facilitate microbial access. Aspergillus niger, known for its cellulolytic capabilities, is used as a microbial agent. The chapter describes the preparation of spore suspensions, substrate sterilization, and inoculation processes to ensure optimal conditions for fungal growth. SSFSolid state fermentation (SSF) is conducted at 30 °C under controlled moisture and humidity conditions, enhancing enzyme–substrate interaction. Cellulase extraction involves buffer-mediated recovery, followed by centrifugation and filtration to isolate crude enzyme. Cellulase activity is quantified using a filter paper assay (FPA), with reducing sugars measured spectrophotometrically via the dinitrosalicylic acid (DNS) method. This methodology maximizes cellulase yield, aligning with sustainable bioresource valorization within the circular bioeconomy framework.