Optogenetics has revolutionized the field of neuroscience by enabling the manipulation of specific neuronal populations with exceptional precision. Here, a robust method is described that integrates optogenetic stimulation of olfactory bulb glomeruli with foot shock fear conditioning. By merging the sophistication of optogenetics with the well-established principles of fear conditioning, this method opens new avenues for exploring the neural dynamics that drive adaptive behavioral responses to olfactory threats.