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The diagnosis and monitoring of neurological disorders are challenging due to their complexity and the difficulty of obtaining samples of damaged tissue while the patient is alive. Cerebrospinal fluid (CSF) is an excellent biofluid for conducting diagnostic and monitoring studies because it is in direct contact with the brain, allowing for the analysis of secreted proteins and peptides associated with various neurological disorders. The Proximity Extension Assay, which combines antibodies with oligonucleotides to detect proteins via qPCR, is revolutionizing the field of biomarker identification. The Olink Target 48 Cytokine Panel is an optimal tool for evaluating the inflammatory profile of CSF samples, requiring only one microliter. The workflow involves incubation with antibody-linked oligonucleotides, extension, amplification through a PCR assay, and detection using microfluidic qPCR, enabling precise and accurate quantification of cytokine levels in pg/mL. This approach offers valuable insights into inflammatory processes within the CSF, serving as a robust tool for studying and understanding inflammation in neurological contexts.

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Monitoring Cerebrospinal Fluid Inflammatory Mediators by Olink Target 48 Cytokine Panel

  • 实验方案

  • 发表日期：

    2025年

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  • Fabricio Zambom-Ferraresi,
  • Fabiola Zambom-Ferraresi,
  • Maite Izco,
  • Nicolás Martínez-Velilla,
  • Leire Extramiana,
  • Joaquín Fernández-Irigoyen,
  • Enrique Santamaría,
  • Adrían Llamas-Urbano,
  • Yasin Hanaee,
  • Julio M. Martinez-Moreno,
  • Nuria Barbarroja,
  • Carlos Pérez-Sánchez

摘要

The diagnosis and monitoring of neurological disorders are challenging due to their complexity and the difficulty of obtaining samples of damaged tissue while the patient is alive. Cerebrospinal fluid (CSF) is an excellent biofluid for conducting diagnostic and monitoring studies because it is in direct contact with the brain, allowing for the analysis of secreted proteins and peptides associated with various neurological disorders. The Proximity Extension Assay, which combines antibodies with oligonucleotides to detect proteins via qPCR, is revolutionizing the field of biomarker identification. The Olink Target 48 Cytokine Panel is an optimal tool for evaluating the inflammatory profile of CSF samples, requiring only one microliter. The workflow involves incubation with antibody-linked oligonucleotides, extension, amplification through a PCR assay, and detection using microfluidic qPCR, enabling precise and accurate quantification of cytokine levels in pg/mL. This approach offers valuable insights into inflammatory processes within the CSF, serving as a robust tool for studying and understanding inflammation in neurological contexts.