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The yellow fever virus (YFV), prototype of the genus Orthoflavivirus (family Flaviviridae), contains a positive-sense RNA genome that is translated as a single polyprotein which comprises approximately 11,000 nucleotides, encoding 3411 amino acids. In recent years, a number of molecular assays have been reported for YFV RNA detection, with different levels of sensitivity and specificity. Reverse transcription Polymerase Chain Reaction (RT-PCR) assays are the most frequent methods for YFV molecular diagnosticsDiagnostics currently. Molecular methods of diagnosticsDiagnostics for viral genome detection offer a fast, sensitive, and highly specific alternative to serological assays for early diagnosticsDiagnostics during the viremic phase of infection or in fatal cases tissues. This chapter presents some conventional RT-PCR protocols used in molecular diagnosticsDiagnostics, among the several available in the literature.

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Conventional RT-PCR for Yellow Fever Virus

  • 实验方案

  • 发表日期：

    2025年

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  • Nieli Rodrigues da Costa Faria,
  • Renata Monteiro-Maia,
  • Priscila Conrado Guerra Nunes,
  • Monique da Rocha Queiroz Lima,
  • Fernanda de Bruycker-Nogueira

摘要

The yellow fever virus (YFV), prototype of the genus Orthoflavivirus (family Flaviviridae), contains a positive-sense RNA genome that is translated as a single polyprotein which comprises approximately 11,000 nucleotides, encoding 3411 amino acids. In recent years, a number of molecular assays have been reported for YFV RNA detection, with different levels of sensitivity and specificity. Reverse transcription Polymerase Chain Reaction (RT-PCR) assays are the most frequent methods for YFV molecular diagnosticsDiagnostics currently. Molecular methods of diagnosticsDiagnostics for viral genome detection offer a fast, sensitive, and highly specific alternative to serological assays for early diagnosticsDiagnostics during the viremic phase of infection or in fatal cases tissues. This chapter presents some conventional RT-PCR protocols used in molecular diagnosticsDiagnostics, among the several available in the literature.