Actinidia deliciosa, commonly known as kiwi, is a woody perennial species recognized for its commercially relevant fruits with high levels of vitamin C, potassium, and dietary fiber. This chapter explores an efficient Agrobacterium-mediated stable transformation method for Actinidia, focusing on A. deliciosa cv Hayward, coupled with a somatic organogenesis regeneration protocol serving as a foundational tool for manipulating the kiwifruit genome. Beyond technical details, this contribution aims to advance both fundamental understanding and practical applications in kiwifruit genetics, providing a valuable resource for researchers.