Scholarlink

The 3DEP Reader (DEPtech) is a commercial noninvasive, high throughput, easily adoptable benchtop microfluidic platform that utilizes the principles of dielectrophoresis to analyze cells in suspension and determine their electrical properties. This protocol describes the operation, value, and common setup of the platform to provide users with the ability to characterize three important electrophysiological parameters of approximately 20,000 cells in under 2 min. Dielectrophoresis (DEP) is an electrokinetic phenomenon which describes the movement of suspended polarizable cells, in a nonuniform electric field. The 3DEP plots the relative DEP force experienced by cells across a range of AC frequencies generating a spectrum through which membrane conductance, membrane capacitance, and cytoplasmic conductivity are derived.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Quantifying the Electrophysiological Properties of Cells Using Dielectrophoresis

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • Krista S. P. Clarke,
  • Oreoluwa V. Griffiths,
  • Kai Hoettges,
  • Rebecca Lewis,
  • Fatima H. Labeed

摘要

The 3DEP Reader (DEPtech) is a commercial noninvasive, high throughput, easily adoptable benchtop microfluidic platform that utilizes the principles of dielectrophoresis to analyze cells in suspension and determine their electrical properties. This protocol describes the operation, value, and common setup of the platform to provide users with the ability to characterize three important electrophysiological parameters of approximately 20,000 cells in under 2 min. Dielectrophoresis (DEP) is an electrokinetic phenomenon which describes the movement of suspended polarizable cells, in a nonuniform electric field. The 3DEP plots the relative DEP force experienced by cells across a range of AC frequencies generating a spectrum through which membrane conductance, membrane capacitance, and cytoplasmic conductivity are derived.