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Nicotinic acid adenine dinucleotide phosphate (NAADP) is a highly potent Ca2+ releasing second messenger with a distinct role during early T cell activation. While studies have proposed NAADP signaling in T cells as a target for treating autoimmune diseases, there are currently no pharmacologic inhibitors available for clinical trial. This is in part due to the fact, that investigating NAADP-evoked Ca2+ signals in T cells currently requires laborious techniques such as microinjections. We have combined recent advances on NAADP signaling to develop a protocol for measuring NAADP-evoked Ca2+ release from permeabilized T cells, that can be used to further characterize this pathway in T cells and validate new pharmacologic agents.

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Measuring NAADP-Evoked Ca2+ Release in Permeabilized T Cells

  • 实验方案

  • 发表日期：

    2025年

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  • Kai J. Winterberg,
  • Andreas Bauche,
  • Ralf Fliegert,
  • Andreas H. Guse

摘要

Nicotinic acid adenine dinucleotide phosphate (NAADP) is a highly potent Ca2+ releasing second messenger with a distinct role during early T cell activation. While studies have proposed NAADP signaling in T cells as a target for treating autoimmune diseases, there are currently no pharmacologic inhibitors available for clinical trial. This is in part due to the fact, that investigating NAADP-evoked Ca2+ signals in T cells currently requires laborious techniques such as microinjections. We have combined recent advances on NAADP signaling to develop a protocol for measuring NAADP-evoked Ca2+ release from permeabilized T cells, that can be used to further characterize this pathway in T cells and validate new pharmacologic agents.