Scholarlink

This chapter presents a detailed protocol for peptide-major histocompatibility complex (pMHC) multimer staining and magnetic activated cell separation, offering a step-by-step guide to isolating and studying low-frequency antigen-specific T cells. This methodological approach provides a means to evaluate the phenotype and function of antigen-specific T cells, potentially facilitating the assessment of novel therapeutic interventions and aiding in understanding T cell exhaustion in chronic viral diseases. Despite challenges such as the necessity of knowing the epitope and HLA restrictions as well as the intricate nature of the pMHC multimer staining process, this protocol serves as a fundamental tool for both basic research and translational applications in chronic viral infections.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Utilizing pMHC Multimers to Study Antigen-Specific T Cells and T Cell Exhaustion in Chronic Viral Infections

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • K. Keetz,
  • M. Knapp,
  • J. Schulze zur Wiesch

摘要

This chapter presents a detailed protocol for peptide-major histocompatibility complex (pMHC) multimer staining and magnetic activated cell separation, offering a step-by-step guide to isolating and studying low-frequency antigen-specific T cells. This methodological approach provides a means to evaluate the phenotype and function of antigen-specific T cells, potentially facilitating the assessment of novel therapeutic interventions and aiding in understanding T cell exhaustion in chronic viral diseases. Despite challenges such as the necessity of knowing the epitope and HLA restrictions as well as the intricate nature of the pMHC multimer staining process, this protocol serves as a fundamental tool for both basic research and translational applications in chronic viral infections.