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Molecular methods permit measurements of viral genomic RNA as well as of the negative-strand template generated during rhinovirus replication. As such, assays of negative-strand RNA can provide a means for relative quantification of levels of double-stranded RNA that is generated during replication and that interacts with pattern recognition receptors to activate the host immune response. Here we provide RT-PCR protocols for quantitative assays for rhinovirus genomic and negative-strand RNAs.

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Quantifying Rhinovirus Genomic and Negative-Strand RNA by RT-PCR

  • 实验方案

  • 发表日期：

    2025年

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  • Shahina Wiehler,
  • Michelle E. Love,
  • David Proud

摘要

Molecular methods permit measurements of viral genomic RNA as well as of the negative-strand template generated during rhinovirus replication. As such, assays of negative-strand RNA can provide a means for relative quantification of levels of double-stranded RNA that is generated during replication and that interacts with pattern recognition receptors to activate the host immune response. Here we provide RT-PCR protocols for quantitative assays for rhinovirus genomic and negative-strand RNAs.