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The advent of proteomics has enabled the identification of individual proteins within seminal plasma. Here, we describe the steps required to isolate seminal plasma from semen and subsequently create a high-quality peptide mixture for bottom-up proteomic analysis using Filter-Aided Sample Preparation.

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Seminal Plasma Proteomics Using Filter-Aided Sample Preparation

  • 实验方案

  • 发表日期：

    2025年

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  • Taylor Pini,
  • Simon P. de Graaf

摘要

The advent of proteomics has enabled the identification of individual proteins within seminal plasma. Here, we describe the steps required to isolate seminal plasma from semen and subsequently create a high-quality peptide mixture for bottom-up proteomic analysis using Filter-Aided Sample Preparation.