Spermatozoa have unique RNA biology compared to the other somatic cells. Therefore, sample collection to final quantification requires an optimized method. Here, we described proper processing methods to quantify sperm mRNA.

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Detection and Quantification of mRNA in Mammalian Spermatozoa

  • Won-Ki Pang,
  • Myung-Geol Pang

摘要

Spermatozoa have unique RNA biology compared to the other somatic cells. Therefore, sample collection to final quantification requires an optimized method. Here, we described proper processing methods to quantify sperm mRNA.