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RNA interference (RNAi) has emerged as an innovative and promising tool for downregulating gene expression, particularly in the context of crop improvement. This advanced technology presents new avenues for developing strategies to knock down and regulate the expression of specific genes, thereby conferring resistance against a variety of plant viruses. This research has uncovered the capacity of double-stranded RNA (dsRNA) molecules to initiate RNAi-mediated gene suppression, offering a means to combat viral diseases in plants and potentially paving the way for the design of RNA vaccines. The threat posed by viruses causing diseases in plants is substantial, impacting global food production and ecological stability. Conventional management systems have proven ineffective in addressing plant viral diseases, necessitating innovative approaches. The concept of RNAi holds significant promise for managing viral diseases in plants. The utilization of the Escherichia coli strain HT115 in producing substantial amounts of dsRNAs represents an in vivo bacterial expression system that is not only effective but also more cost-efficient compared to the in vitro transcription method.

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In Vivo Synthesis and Purification of Double-Stranded RNA (dsRNA) for RNA Interference (RNAi)

  • 实验方案

  • 发表日期：

    2025年

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  • Wani Farhana,
  • Nulevino Iralu,
  • Sumiah Wani,
  • Bikash Mandal,
  • Aflaq Hamid

摘要

RNA interference (RNAi) has emerged as an innovative and promising tool for downregulating gene expression, particularly in the context of crop improvement. This advanced technology presents new avenues for developing strategies to knock down and regulate the expression of specific genes, thereby conferring resistance against a variety of plant viruses. This research has uncovered the capacity of double-stranded RNA (dsRNA) molecules to initiate RNAi-mediated gene suppression, offering a means to combat viral diseases in plants and potentially paving the way for the design of RNA vaccines. The threat posed by viruses causing diseases in plants is substantial, impacting global food production and ecological stability. Conventional management systems have proven ineffective in addressing plant viral diseases, necessitating innovative approaches. The concept of RNAi holds significant promise for managing viral diseases in plants. The utilization of the Escherichia coli strain HT115 in producing substantial amounts of dsRNAs represents an in vivo bacterial expression system that is not only effective but also more cost-efficient compared to the in vitro transcription method.