Recombinase polymerase amplification (RPA) offers a rapid, sensitive, and isothermal approach for detecting viral infections in plants. Unlike traditional PCR methods, RPA operates at a constant low temperature of approximately 37 °C, eliminating the need for sophisticated thermal cyclers. This study outlines the application of RPA for the detection of plant viruses, highlighting its efficiency and simplicity. Plant tissue samples were collected and viral RNA/DNA was extracted using a plant nucleic acid extraction kit. The RPA reaction was prepared with specific primers for the target virus, along with the necessary enzymes and buffers. For RNA viruses, a reverse transcriptase enzyme was included. The reactions were incubated at 37–42 °C for 20–40 min, depending on the viral target. Detection of the amplified products was achieved through various methods, including agarose gel electrophoresis, lateral flow assays, and fluorescence-based detection systems. The results demonstrated that RPA is a versatile and effective tool for the rapid detection of plant viruses, providing a valuable alternative to conventional molecular diagnostic techniques.