RNA extraction from plants, particularly perennials, is sometimes difficult due to the presence of interfering chemicals such as polysaccharides and polyphenolics. The CTAB method is widely used for separating nucleic acids from plant material. However, this method necessitates customization to certain plant species and tissues. The modified CTAB approach applies to a wide variety of woody and herbaceous plants, but this approach includes higher concentrations of β-mercaptoethanol. This technique produced high-quality RNA with low degradation. The extracted RNA is useful for further transcriptional analysis, such as RT-qPCR. The modified CTAB method presented here is a reliable and effective method for isolating RNA from woody plants, enabling molecular genetic research of these economically important species.