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Patch-clamp recordings are a fundamental technique in neuroscience to study the electrical activity of individual cells. When applied to isolated neural cells, acute tissue slices, or brain rosettes, this method provides valuable insights into neuronal function and network activity in different experimental models. Here, we describe a detailed protocol to perform patch-clamp recording in those cell preparations.

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Patch-Clamp Recordings in Isolated Neural Cells, Acute Tissue Slices, and Brain Rosettes

  • 实验方案

  • 发表日期：

    2025年

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  • Marina Arribas-Blázquez,
  • Celia Llorente-Sáez,
  • Luis A. Olivos-Oré,
  • Antonio R. Artalejo

摘要

Patch-clamp recordings are a fundamental technique in neuroscience to study the electrical activity of individual cells. When applied to isolated neural cells, acute tissue slices, or brain rosettes, this method provides valuable insights into neuronal function and network activity in different experimental models. Here, we describe a detailed protocol to perform patch-clamp recording in those cell preparations.