Foodborne disease stands as a significant public health concern, causing millions of infections worldwide each year. The harmful effects of foodborne pathogens on human health highlight the critical relevance of food safety throughout all stages of production. Conventional methods for detecting foodborne pathogens, such as HPLC, molecular approaches, and live culture, have intrinsic limitations such as being laborious, expensive, and time-consuming. These constraints make them unsuitable for on-site applications, demanding the investigation and implementation of more efficient and simplified procedures for the detection of foodborne pathogens. In recent times, there has been a notable focus on lateral flow assays (LFA) due to their capacity for swift, cost-effective, and on-site detection of pathogens. Lateral flow assays are widely used in both qualitative and quantitative assessments across many areas. Their adaptability, variety of formats, and remarkable features make them a popular choice for point-of-care applications. These applications encompass the testing of toxins, hormones, drugs, pathogens, allergens, metabolites, and other pertinent entities. This chapter probes into the methodologies implemented for the detection of Gram-positive bacterial toxins associated with foodborne illnesses through the utilization of LFA. Additionally, it explores the diverse applications of this approach in the sector of food safety.