Scholarlink

Sequencing trait-associated mutations (STAM) is a simple and straightforward gene cloning method that was developed in wheat. It uses full-length isoform sequencing (Iso-Seq) of the wild type as the reference and employs transcriptome sequencing of multiple, independently derived mutants for gene cloning. The STAM method eliminates the need for fine-mapping or a high-quality whole genome assembly of a specific wheat cultivar, and it could also be used in other plant species with complex genomes. Detailed, bioinformatic analysis protocol and tips for STAM are provided in this chapter.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Sequencing Trait-Associated Mutations (STAM) to Clone Rust Resistance Genes

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • Fei Ni,
  • Yang Yu,
  • Lynn Epstein,
  • Daolin Fu,
  • Jiajie Wu

摘要

Sequencing trait-associated mutations (STAM) is a simple and straightforward gene cloning method that was developed in wheat. It uses full-length isoform sequencing (Iso-Seq) of the wild type as the reference and employs transcriptome sequencing of multiple, independently derived mutants for gene cloning. The STAM method eliminates the need for fine-mapping or a high-quality whole genome assembly of a specific wheat cultivar, and it could also be used in other plant species with complex genomes. Detailed, bioinformatic analysis protocol and tips for STAM are provided in this chapter.