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The marker-assisted breeding (MAB) approach has become more convenient and time efficient with the advancement of allele-specific, cost-effective, and breeder-friendly single nucleotide polymorphism (SNP)-based kompetitive allele-specific (KASP) polymerase chain reaction (PCR) markers. Here, we describe a detailed protocol to develop KASP assays for rust resistance breeding in wheat.

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Developing Breeder-Friendly KASP Markers for Rust R Genes

  • 实验方案

  • 发表日期：

    2025年

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  • Jyoti Saini Sharma,
  • Lovepreet Singh

摘要

The marker-assisted breeding (MAB) approach has become more convenient and time efficient with the advancement of allele-specific, cost-effective, and breeder-friendly single nucleotide polymorphism (SNP)-based kompetitive allele-specific (KASP) polymerase chain reaction (PCR) markers. Here, we describe a detailed protocol to develop KASP assays for rust resistance breeding in wheat.