Hydration is essential for athletic performance and overall health, yet many athletes face challenges in achieving it. A cross-sectional study was conducted on the Ankaragücü youth soccer team in Turkey to assess hydration status. Twenty-six male athletes (15 ± 1.2 years) participated, with data collected over 3 days, including body weight, fluid volume changes, and urinalysis. Hydration was assessed by urine specific gravity (USG), osmolality, and urine color. Results showed a mean daily fluid consumption of 2780 ± 567 mL, of which 908.6 ± 332.7 mL was consumed during training. Dehydration levels averaged 0.5% over the two training days. Urinalysis showed a strong correlation between USG (strip) with both USG (laboratory) and USG (refractometry) (r = 0.8; P < 0.01) and with osmolality (r = 0.7; P < 0.01), while its association with urine color was moderate (r = 0.4; P < 0.05). Despite adequate hydration, some athletes completed training in a dehydrated state, highlighting the need for improved hydration strategies. This study highlights the effectiveness of urine strips for practical, field-based hydration assessment and emphasizes the importance of hydration education for young athletes to prevent dehydration and enhance performance.