Wheat blast, caused by the Magnaporthe oryzae pathotype Triticum (MoT), is a devastating fungal disease that ravages wheat crops, threatening the world’s food security. Effective management requires a rapid, precise, and field-deployable diagnostic approach for wheat blast pathogen identification, given the unreliability of visual symptom-based treatment. Current diagnostic strategies, such as enzyme-linked immunosorbent assays, polymerase chain reaction, and genome sequencing, demand sophisticated equipment, sterile conditions, and skilled personnel, rendering them impractical for extensive on-site use. To address this challenge, we developed a highly accurate approach for detecting MoT in the seedling, vegetative and ripening stages of wheat by harnessing CRISPR technology. In this chapter, we present the protocol for a rapid and exceptionally accurate method for MoT detection that combines streamlined DNA extraction techniques, recombinase polymerase for amplification, Cas12a for target recognition, and a commercially available lateral flow assay kit for result visualization. This rapid method can be deployed in early detection of wheat blast fungus in the field for surveillance and also in quarantine offices for the management and to limit the further spread of this wheat killer via seeds.