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Functionalized lipid analogs are a versatile tool for studying lipid localization, metabolism and interactions in cells. As a common follow-up research, in vitro experiments are utilized to confirm specific interactors and characterize protein-lipid interactions. Here, we describe protocols that rely on commercially available photoactivatable and clickable lipids in reconstituted systems to capture protein-lipid interactions and detect lipid transport between purified proteins.

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In Vitro Approaches for Detection of Protein-Lipid Interactions and Protein-to-Protein Transport of Lipids Using Bifunctional Lipid Analogs

  • 实验方案

  • 发表日期：

    2025年

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  • Denisa Jamecna,
  • Doris Höglinger

摘要

Functionalized lipid analogs are a versatile tool for studying lipid localization, metabolism and interactions in cells. As a common follow-up research, in vitro experiments are utilized to confirm specific interactors and characterize protein-lipid interactions. Here, we describe protocols that rely on commercially available photoactivatable and clickable lipids in reconstituted systems to capture protein-lipid interactions and detect lipid transport between purified proteins.