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Co-culture assays are used to study the mutual interaction between cells in vitro. This chapter describes 2D and 3D co-culture systems used to study cell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most used cell culture techniques, functional assays, and gene expression.

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2D and 3D In Vitro Co-culture for Cancer and Bone Cell Interaction Studies

  • 实验方案

  • 发表日期：

    2025年

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  • Ryan T. Bishop,
  • Jesus Delgado-Calle,
  • Michaela R. Reagan,
  • Silvia Marino

摘要

Co-culture assays are used to study the mutual interaction between cells in vitro. This chapter describes 2D and 3D co-culture systems used to study cell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most used cell culture techniques, functional assays, and gene expression.