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This chapter is a revised version of the first Edition which aims to describe a method used to evaluate gene expression and microRNAs (miRNAs) in bone cells or bone tissue using Reverse transcription and quantitative Polymerase Chain Reaction (RT-qPCR), and methods to assess chromogenic in situ hybridization (CISH) on Formalin Fixed Paraffin Embedded (FFPE) mouse bone tissue to detect both DNA and mRNA transcripts using the double digoxigenin (DIG) locked nucleic acid (LNA™) probes or using RNAscope technology.

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Analysis of mRNA, miRNA, and DNA in Bone Cells by RT-qPCR and In Situ Hybridization

  • 实验方案

  • 发表日期：

    2025年

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  • Morgane Lallier,
  • Emma Racineau,
  • Jérôme Amiaud,
  • Bénédicte Brounais-Le Royer,
  • Steven Georges,
  • Marc Baud’huin,
  • Franck Verrecchia,
  • Benjamin Ory,
  • Francois Lamoureux

摘要

This chapter is a revised version of the first Edition which aims to describe a method used to evaluate gene expression and microRNAs (miRNAs) in bone cells or bone tissue using Reverse transcription and quantitative Polymerase Chain Reaction (RT-qPCR), and methods to assess chromogenic in situ hybridization (CISH) on Formalin Fixed Paraffin Embedded (FFPE) mouse bone tissue to detect both DNA and mRNA transcripts using the double digoxigenin (DIG) locked nucleic acid (LNA™) probes or using RNAscope technology.