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This chapter describes the analysis of signaling pathways in bone cells by the use of Western blotting and immunoprecipitation, including a step-by-step guide to cell culture techniques, cellular and subcellular fractionation, protein isolation, purification, measurement, electrophoretic transfer, and detection.

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Analysis of Signaling Pathways by Western Blotting and Immunoprecipitation

  • 实验方案

  • 发表日期：

    2025年

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  • Feier Zeng,
  • Silvia Marino,
  • Aymen I. Idris

摘要

This chapter describes the analysis of signaling pathways in bone cells by the use of Western blotting and immunoprecipitation, including a step-by-step guide to cell culture techniques, cellular and subcellular fractionation, protein isolation, purification, measurement, electrophoretic transfer, and detection.