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Recent work in single-cell imaging has allowed unprecedented insight into single-cell interactions that control disease progression. However, approaches to understanding the combined extracellular and cellular microenvironment are limited. In the current protocol, we describe an approach that allows single-cell type imaging using matrix-assisted laser desorption/ionization immunohistochemistry (MALDI-IHC) of UV (ultraviolet) photocleavable mass tags combined with N-glycomic and ECM-targeted proteomic imaging from the same formalin-fixed paraffin-embedded tissue section. These approaches use the same imaging mass spectrometry platform to provide a comprehensive view of both the cellular and extracellular tissue microenvironment.

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Multiplexed and Multiomic Mass Spectrometry Imaging of MALDI-IHC Photocleavable Mass Tag Tissue Probes, N-Glycomics, and the Extracellular Matrisome from FFPE Tissue Sections

  • 实验方案

  • 发表日期：

    2025年

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  • Harrison B. Taylor,
  • Jaclyn B. Dunne,
  • Mark J. Lim,
  • Gargey Yagnik,
  • Kenneth J. Rothschild,
  • Anand Mehta,
  • Richard R. Drake,
  • Peggi M. Angel

摘要

Recent work in single-cell imaging has allowed unprecedented insight into single-cell interactions that control disease progression. However, approaches to understanding the combined extracellular and cellular microenvironment are limited. In the current protocol, we describe an approach that allows single-cell type imaging using matrix-assisted laser desorption/ionization immunohistochemistry (MALDI-IHC) of UV (ultraviolet) photocleavable mass tags combined with N-glycomic and ECM-targeted proteomic imaging from the same formalin-fixed paraffin-embedded tissue section. These approaches use the same imaging mass spectrometry platform to provide a comprehensive view of both the cellular and extracellular tissue microenvironment.