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Molecular motors move processively along cytoskeletal filaments by stepping of their motor domains (MDs). Observation of how the MDs step relative to each other reveals the mechanism of motor processivity and various gating mechanisms used by motors to coordinate the catalytic cycles of their MDs. This chapter will discuss developments in simultaneous observation of the stepping motions of the two MDs of processive motors using two-color single-particle tracking microscopy. Techniques presented: FIONA, multicolor tracking/image registration.

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Multicolor Tracking of Molecular Motors at Nanometer Resolution

  • 实验方案

  • 发表日期：

    2025年

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  • Joseph Slivka,
  • Ahmet Yildiz

摘要

Molecular motors move processively along cytoskeletal filaments by stepping of their motor domains (MDs). Observation of how the MDs step relative to each other reveals the mechanism of motor processivity and various gating mechanisms used by motors to coordinate the catalytic cycles of their MDs. This chapter will discuss developments in simultaneous observation of the stepping motions of the two MDs of processive motors using two-color single-particle tracking microscopy. Techniques presented: FIONA, multicolor tracking/image registration.