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Neutralizing antibodies play an important role in protection against filoviruses. However, the technique to assess neutralization titers with a replicating BSL-4 virus is limited. This chapter explains how to determine the neutralization titer in serum samples against Marburg virus (MARV). This method uses a replicating recombinant vesicular stomatitis virus expressing the MARV glycoprotein and the green fluorescent protein as a surrogate approach.

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Quantification of Neutralizing Antibodies in Serum Using VSV-MARV-GFP

  • 实验方案

  • 发表日期：

    2025年

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  • Paige Fletcher

摘要

Neutralizing antibodies play an important role in protection against filoviruses. However, the technique to assess neutralization titers with a replicating BSL-4 virus is limited. This chapter explains how to determine the neutralization titer in serum samples against Marburg virus (MARV). This method uses a replicating recombinant vesicular stomatitis virus expressing the MARV glycoprotein and the green fluorescent protein as a surrogate approach.