Hypothyroidism, which is characterized by insufficient production of thyroid hormones, and malaria, a mosquito-borne infectious disease caused by Plasmodium parasites, are significant global health challenges. Studying how these two conditions interact could provide valuable insights into their complex relationship and potential treatment options. To induce hypothyroidism in the research, scientists used drugs to block the production of thyroid hormones. Then, they infected mice with Plasmodium berghei ANKA to mimic cerebral malaria infection. The spleen is essential in the body’s immune response to malaria. It is involved in both innate and adaptive immunity, iron recycling, and the removal of old red blood cells or damaged cells infected with Plasmodium. Monitoring disease progression in male mice is crucial for early detection, and techniques like flow cytometry can help identify specific immune system populations within the spleen that are relevant to the research.