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Live imaging of microtubules (MTs) allows obtaining mechanistic insights into cell division. In literature, mitotic spindle dynamics have been investigated in mammalian systems largely focusing on established cell lines. Here, we describe a detailed protocol that investigates MT dynamics during cell division in a 3D mouse intestinal organoid model that more accurately captures the in vivo system.

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High-Resolution Imaging of Spindle Orientation Dynamics in 3D Intestinal Organoids

  • 实验方案

  • 发表日期：

    2025年

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  • Amlan Barai,
  • Delphine Delacour

摘要

Live imaging of microtubules (MTs) allows obtaining mechanistic insights into cell division. In literature, mitotic spindle dynamics have been investigated in mammalian systems largely focusing on established cell lines. Here, we describe a detailed protocol that investigates MT dynamics during cell division in a 3D mouse intestinal organoid model that more accurately captures the in vivo system.