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In metaphase, chromosomes undergo a back-and-forth movement between two spindle poles called chromosome oscillation. This dynamic is necessary to maintain the robustness of chromosome segregation. This chapter describes the materials and methods required to observe chromosome oscillation in mammalian cell lines and calculate the deviation of amplitude (DAP), which is used to quantify the degree of chromosome oscillation.

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Assessment of Chromosome Oscillations in Mammalian Cells by Live Cell Imaging

  • 实验方案

  • 发表日期：

    2025年

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  • Kenji Iemura,
  • Kozo Tanaka

摘要

In metaphase, chromosomes undergo a back-and-forth movement between two spindle poles called chromosome oscillation. This dynamic is necessary to maintain the robustness of chromosome segregation. This chapter describes the materials and methods required to observe chromosome oscillation in mammalian cell lines and calculate the deviation of amplitude (DAP), which is used to quantify the degree of chromosome oscillation.