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As we exploit biological machineries and circuits to redesign nature, it is just important to use efficient cloning strategies and methods to heterologously express the resulting DNA constructs. Golden Gate cloning allows the assembly of multiple fragments in a single reaction, making the process efficient and seamless. Although Golden Gate strategies have already been employed for different organisms, it is still not well-established for Actinobacteria. Here, we describe methods for Golden Gate cloning and how it can be utilized for Actinobacteria.

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Golden Gate Cloning in Actinobacteria: Opportunities and Challenges

  • 实验方案

  • 发表日期：

    2025年

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  • Anna Christina R. Ngo,
  • Melody Haarmann,
  • Nils Weindorf,
  • David Angelo V. Guanzon,
  • Vivian Linke,
  • Joe Smitka,
  • Dirk Tischler

摘要

As we exploit biological machineries and circuits to redesign nature, it is just important to use efficient cloning strategies and methods to heterologously express the resulting DNA constructs. Golden Gate cloning allows the assembly of multiple fragments in a single reaction, making the process efficient and seamless. Although Golden Gate strategies have already been employed for different organisms, it is still not well-established for Actinobacteria. Here, we describe methods for Golden Gate cloning and how it can be utilized for Actinobacteria.