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Phosphorylation of FOXO transcription factors is one of the key mechanisms involved in the regulation of the activity, nucleo-cytosolic shuttling, and stability of this family of proteins. Here we describe several experimental approaches allowing analysis of changes in the phosphorylation of these proteins upon exposure to different stimuli.

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Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate Their Activity

  • 实验方案

  • 发表日期：

    2025年

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  • Alba Orea-Soufi,
  • David Dávila,
  • María Salazar-Roa,
  • Mar Lorente,
  • Guillermo Velasco

摘要

Phosphorylation of FOXO transcription factors is one of the key mechanisms involved in the regulation of the activity, nucleo-cytosolic shuttling, and stability of this family of proteins. Here we describe several experimental approaches allowing analysis of changes in the phosphorylation of these proteins upon exposure to different stimuli.